Asian Journal of Pharmacy and Technology

S. Gnanavel ¹, R. Bharathidasan ¹, R. Mahalingam ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 BG and Research Dept of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The antimicrobial effect of some selected Indian medicinal plants Strychnos nux-vomica and Cassia angustifolia, were selected. The solvents used for the extraction of plant leaves were n-butanol, methanol and distilled water. The invitro antimicrobial activity was performed by agar well diffusion method. The most susceptible gram-positive bacteria was Staphylococcus and Salmonella while other most susceptible gram negative bacteria was klebsiella pneumonia and fungal species namely Aspergillus terreus, Aspergillus flavus and Aspergillus niger. The n-butanol extract of plant Strychnos nux-vomica and Cassia angustifolia, inhibit all the bacterial and fungal strains investigated. The most active extract was compared with the standard antibiotics, penicillin, streptomycin and ampicillin with 100mg disc. In the present study Strychnos nuxvoimica and Cassia angustifolia showed maximum inhibition against the fungal and bacterial organisms tested. Thus the n-butanol plant extracts could be used to control the above microbes.

Full Text

     

K. Kaviyarasi ¹, K. Kanimozhi ¹, P. Madhanraj ², A. Panneerselvam ¹, V. Ambikapathy ¹

Affiliations
1 Department of Botany and Microbiology, A.V.V.M .Sri Puspham College [Autonomous], Poondi-613 03, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621 212, Tamil Nadu, IN

Abstract

The soil sample was collected from Manora. After analysing the physic-chemical parameters of the collected marine soil sample, ten actinomycetes species including Actinobispora yunnanensis, Streptomyces albus,Micromonospora echinospora, Saccharopolyspora hirsute, Streptomycetes cyaneus, Actinomadura citrea, Saccharomonospora viridis, Thermomonospora mesophila, Streptoverticillium album Microtetrospora fastidiosa were isolated and they were screened for phosphate solubilization using Pikovskaya's agar medium and the Actinobispora yunnanensis was found to be dominant in phosphate solubilization. Then the DNA of the Actinobispora yunnanensis was isolated, amplified and its 16S rDNA was sequenced and its secondary structure was also predicted.The phylogenetic analysis of the Actinobispora yunnanensis was also carried out.

Keywords

Phosphate, Phosphate Solubilization, 16S r DNA, Actinobispora yunnanensis.

Full Text

     

A. Gayathri ¹, P. Madhanraj ², A. Panneerselvam ¹

Affiliations
1 Department of Botany and Microbiology, A. V. V. M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN

Abstract

The present study was to isolate and identify antimicrobial activity producing actinomycetes strains from marine habitat, to find out antibacterial efficiency against the common human pathogens. Marine actinomycetes were isolated from salt pan region of Kodiakarai, Nagapattinam [District].Out of 20 isolated actinomycetes 10 were identified and selected for antibacterial activity. Out of 10 dominant actinomycete species Streptoverticillium album was highly dominant and showed the best level of antibacterial activity against three human pathogens, Staphylococcus aureus, Klebsiella pheumoniae and Escherichia coli. The isolation, characterization and the study on actinomycetes can be useful in the discovery of antibiotics and also confirmed by molecular characterization.

Keywords

Antibacterial Activity, Sterptoverticillium album, Marine Actinomycetes.

Full Text